In order to study the vertical vibration of large-diameter end-bearing pile in layered soils, we first modified the classical soil vibration model. Then the effects of plasticity index and the strain of soil around the pile on dynamic shear stress, shear modulus and hysteretic damping ratio of the soil were further studied. To solve the dynamic impedance of soil under nonlinear condition, soil around the pile was divided into different sections. The pile-soil vibration model of large diameter end bearing pile in layered soil was obtained by the transfer matrix method. Finally, the influencing factors and changing rules of dynamic impedance of pile bottom were obtained. The results showed that the plasticity index, strain of soil around the pile, pile-soil relative stiffness and slenderness ratio have great impacts on the dynamic impedance of the large diameter pile when the soil nonlinearity was considered, which should be considered in the engineering design.