In light of the situation that the influence of human activities on groundwater quality is more and more serious the paper took comprehensive water quality assessment of Dong-E groundwater source for example , combined with matlab and introduced time series analysis theory to analyze the series of monitoring value of water quality.The results show that total hardness monitoring series jumped in early 2013 and then kept rising. There was not periodic component.There was unreasonable and random mining actives which leaded to the increase of total hardness content in early 2013,but there was not periodical mining actives which affected the change of total hardness.In addition, this article also made short-term prediction based on the analysis.The result showed that if the management of water quality is not strengthed，the total hardness content will reach 451 mg/L in early 2018 which means Ⅳ-class water quality.The paper can offer new idea for the comprehensive assessment of groundwater quality and provide technical support for the assessment of groundwater ecological environment and management of groundwater source.