To analyze regional differences of flow regimes in the Yellow River Basin, the Mann-Kendall test method was adopted to explore the temporal variation trend and Range of Variability Approach (RVA) was applied to assess hydrological alteration of metrics at all stations from 1960 to 2015. The results show that: Except for the Huangheyan station, most flow regime metrics of the Tangnaihai station and other stations in the upper, middle and lower reaches show a decreasing trend, especially monthly flow in the flood season, high flow and its duration. And the annual maximum flow in the upper, middle and lower reaches also decreases significantly; Some metrics also have regional differences. For example, the monthly runoff of June and September and maximum runoff of the Huangheyan station increase significantly; the occur time of minimum flow is delayed; the frequency of high flow in the middle reaches decreases significantly, and the occur time of maximum time is significantly advanced; the monthly runoff in downstream is significantly reduced, and the occur time of maximum time is significantly advanced; The flow regimes of hydrological stations in the Yellow River Basin have been completely changed. The overall degree of change is moderate and above, and the frequency and duration of changing degree are the greatest. Among them, Lijin Station has the largest changing degree; followed by Longmen Station.