Rural wastewater treated by soil filtration system in greenhouse was used for drip irrigation, and the spatial and temporal variation rules of Chemical oxygen demand CODCr, biochemical oxygen demand BOD5, total nitrogen TN, ammonia nitrogen NH3—N and total phosphorus TP in the soil and seepage after irrigation were analyzed. A set of negative pressure meters was buried at a depth of 20 cm directly below the drop head in the test area. Irrigation was carried out when the soil substrate potential indicated by the negative pressure meter was lower than -25kPa, and the irrigation volume was 5 mm each time. The experimental results showed that the concentration decreased with depth. The concentrations of substances in the deep seepage at the different seasons were successively from high to low ranked: summer > autumn > spring > winter. The maximum removal rates of CODCr, BOD5, TN, NH3—N and TP were found to be 76.3%-88.1%, 84.2%-89.2%, 69.3%-76.8%, 57.3%-63.1% and 69.8%-78.6%, respectively, in the deep seepage at a depth of 1.5m. At the outer edge of the drip irrigation area, the attenuation rate of each substance increases gradually along the horizontal direction of water flow. Using agricultural wastewater regenerated drip irrigation, the concentration of TN and TP in deep seepage was low. By regulating the concentration of regenerated wastewater, pollutants can be controlled to flow into shallow groundwater to prevent non-point source pollution.