水资源与水工程学报

董艳荣，狄军贞，胡海洋，周君厚，阮浈，孙雪莹，赵中茜.硫酸盐还原菌分离及其处理煤矿酸性废水工艺的实验研究水资源与水工程学报[J].,2019,30(2):25-30

硫酸盐还原菌分离及其处理煤矿酸性废水工艺的实验研究

Experimental study in isolation of sulfate reducing bacteria and treatment of acid mine drainage process

DOI：10.11705/j.issn.1672-643X.2019.02.05

中文关键词: 硫酸盐还原菌煤矿酸性废水工艺优化 Fe²⁺去除 SO₄^2-去除

英文关键词:sulfate-reducing bacteria acid mine drainage process optimization Fe²⁺ removal SO₄^2- removal

基金项目:国家自然科学基金项目(41672247)；国家级大学生创新训练项目(201710147000235)；辽宁省教育厅科学技术研究项目(LJ2017FAL016)

作者	单位
董艳荣，狄军贞，胡海洋，周君厚，阮浈，孙雪莹，赵中茜	(辽宁工程技术大学土木工程学院，辽宁阜新 123000)

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中文摘要:

针对煤矿酸性废水直接排放污染水资源的问题，试验选用污泥样作为筛选样品富集硫酸盐还原菌，采用稀释涂布-叠皿夹层厌氧培养法分离纯化SRB，研究了SRB对煤矿酸性废水中Fe²⁺和SO₄^2-的去除效果。试验结果表明：分离纯化得到的SRB符合典型的“S”型生长曲线，SRB的最佳生长条件为34℃、pH为7、70%乳酸钠作为碳源。综合比较接种不同量SRB对煤矿酸性废水的处理效果可知，接种10% SRB的处理效果较好，接种时间为5d时对煤矿酸性废水中SO₄^2-和Fe²⁺的去除率分别为74.71%和99.18%。

英文摘要:

Aiming at solving the problem of the water pollution by acid waste water discharged directly from the coal mine, this study enriched sulfate-reducing bacteria (SRB) from river sludge as a screening sample, separated and purified SRB using dilution coating-lamination plate anaerobic culture method, and studied the removal effects of Fe²⁺ and SO₄^2- in acid mine drainage from coal mine by SRB. The results showed that the SRB obtained from the isolate coincided with the typical “S” growth curve. The optimum growth conditions of SRB was 34 ℃, pH was seven, and 70% sodium lactate was added as carbon source. The treatment effect on acid mine drainage from coal mine of 10% SRB was optimum by comprehensive comparison of SRB different inoculation amount. The removal rates of SO₄^2- and Fe²⁺ in coal mine wastewater were 74.71% and 99.18%, respectively, when the inoculation time was five days.

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