The Awati Irrigation District is located on the northwestern edge of the Tarim Basin. The climate is extremely arid with low precipitation and strong evaporation. In recent years, due to a large number of land reclamation, irrational use of water resources, and soil salinization, the ecological environment in irrigated areas become increasingly fragile and hindered its sustainable economic development. Based on the field investigation and analysis of Xinjiang's statistical data of many years, this paper considers the ecological water safety of irrigation districts, and adopts the appropriate ecological water requirements such as Penman formula, quota method and ratio method for different vegetation ecological water demand characteristics to calculate its ecological water demand. The results showed that the total ecological water demand in the Awati Irrigation Area is 13.37×108 m3, of which the ecological water requirement outside the river is 6.8×108 m3, and the ecological water requirement in the river is 6.57×108 m3. Through the ecological water demand studies, the water use recommendations are proposed to provide a theoretical basis for the health and stability of the Awati irrigation area ecosystem.