According to the measurement of Jiangba Station in Shengjin Lake, Huangpen sluice (upper and low reaches) and Anqing gauge station for the period of 2015 to 2017, the influence of water control sluice on water level and lake area for Yangtze River connected lakes was investigated. The main conclusions are: Due to the influence of man made dam interception and water control of Huangpen sluice, the water level of Jiangba Station remained at about 11 m, far higher than other water level stations in dry season. During the wet season, the water level reminds lower than the warning water level of 15 m, which is consistent with the upper of Huangpen sluice fluctuation, expect for the extraordinary flood, causing the rivers and lakes connected, and the water level is not controlled by the sluice. The water level of the upper of Huangpen sluice fluctuates between Jiangba Station and the low reaches of the sluice, which was basically in accordance with that of Jiangba Station during the flat water period and the wet season when the water level is higher than 11 m. The water level of the low reaches of the sluice was between the upper sluice and Anqing station during the dry season, which is consistent with water level fluctuation of the Yangtze River. The average monthly area changes of Shengjin Lake under the conditions of natural connection of river and lake and Huangpen sluice control are estimated and compared respectively, which shows that the controlled sluice resulted in the enlargement of lake area in dry season and the Shrinkage of lake area in wet season.