In Shihezi area, groundwater is the main water resource of residents' drinking and domestic water, agriculture irrigation and industrial manufacture. This study aimed to investigate the spatial distribution characteristics and influence factors of ammonia-N, nitrate-N and nitrite-N in groundwater. The study is of great significance to control and govern groundwater pollution, ensuring access to water security in Shihezi area. Based on software of SPSS23, Sufer8 and Mapgis67, the study analyzed the spatial distribution characteristics and influence factors of ammonia-N, nitrate-N and nitrite-N in groundwater. The results showed that 43 groundwater samples of ammonia-N, nitrate-N and nitrite-N reached Ⅲ class standard limit of Groundwater Quality Standard (DZ/T0290-2015). The spatial distribution characteristics of ammonia-N, nitrate-N and nitrite-N contents in the study area were significant. In horizontal direction of the study area, centered on the Shihezi urban area, contents of ammonia-N, nitrate-N and nitrite-N in groundwater gradually decreased to the boundaries around the study area; the contents of ammonia-N, nitrate-N and nitrite-N in the south were higher than that in the north generally. In vertical direction of the study area, nitrate-N content in shallow confined groundwater was higher than that in deep confined groundwater, but lower than that in unconfined groundwater. Contents of nitrite-N and ammonia-N in deep confined groundwater were higher than that in shallow confined groundwater, but lower than that in unconfined groundwater. The sampling points of maximum content of ammonia-N, nitrate-N and nitrite-N were all located in unconfined groundwater. The contents of ammonia-N, nitrate-N and nitrite-N were affected by natural factors and human factors. The natural factors included oxidation-deoxidization environment, acid-alkali environment, depth of unconfined groundwater, aeration zone and aquifer lithology; human factors included land utilization patterns, agricultural pollution, domestic pollution, animal husbandry pollution and agricultural products processing pollution, etc.