This study is based on the measured streamflow data from 1956-2008 from 26 hydrometric stations across Huaihe River Basin (HRB) . The Mann-Kendall, Sequential Mann-Kendall and distribution-free CUSUM tests were employed to detect the trends of streamflow and the abrupt change points of annual streamflow at different spatial scales. The results showed that: except in the headwater catchment of the basin with an insignificant increasing trend, the annual streamflow decreased across the basin, especially in the mainstreams of the northeast part of the Huaihe River System (HRS) and the Yishusi River System (YRS); most stations in the HRS showed decreasing streamflow in spring (i.e. April to May) and rising streamflow in summer (i.e. July and August), indicating that drought and flood in the HRS could be more serious; the decreasing streamflow trend in summer was the strongest as compared to other seasons in the YRS, and the streamflow in July reached the lowest, with 95% significant level. affected by strong human activities, the change points of annual streamflow was relatively complex and shows spatial and temporal variability, wich mainly happened between 1980~1990.