In the light of influence of the worse construction environment and curing condition on work performance of polyurethane sealant,the paper used the laboratory tests, artificially controled temperature,and tested low temperature, process of freezing and thawing, curing at low temperature and primer measure encountered in the construction of sealing joints of concrete lining open channel. It Investigated and analyzed the indicators such as the damage form of sealing material, bond strength and elongation at break.The test results show that when polyurethane sealant meet with the process of freezing and thawing or curing at low temperature,the work performance of polyurethane sealant will have the decline of different degrees.If polyurethane sealant is in damp environment,the effect of Solidification and freezing thawing on the bond trength of polyurethane sealant is very significant,which should be paid more attention in actual engineering. Under special construction environment,to use primer can improved significantly the work performance of sealing material.