The analysis of the change rule of land surface temperature has important significance for the study of hydrothermal change and the drought monitoring in grassland region. The paper inversed the land surface temperature of different land use types based on Mono-window Algorithm on the typical grassland. The results show that the average value of land surface temperature is about 37℃ at the satellite transit time. Compared with the other land use types, the land surface temperature is lower in water body and the wet region.The paper analyzed the sensibility of land surface temperature on the change of two factors by setting up the different change scenarios of two parameters in order to probe the influence of NDVI and atmospheric average temperature on land surface temperature.The result shows that the change of atmospheric average temperature has greater impact on the land surface temperature, and the influence of NDVI on relative error of surface temperature is lower.