The water footprint analysis of agriculture and husbandry can reveal connection and contradiction between agriculture and husbandry economic system and water resources consumption,and provide reference for rational utilization of agricultural water resources and agricultural structure adjustment. In this paper, the water footprint of agriculture and husbandry in Turkmenistan was investigated by method of water footprint. The gross volume of agriculture and husbandry water footprint, structure, population density, land density, utilization degree of water resources, carrying capacity index 2012 and its water resource ecological security were analyzed. The results showed that in general, the agriculture and husbandry gross water footprint is between 1.63×1010~2.73×1010 m3, and basically presents increasing trend every year,the greatest increment is in 2008; the economic crops in water footprint of Turkmenistan account for 67.93% of gross agriculture products; annual dynamic characteristics of population and land density of agriculture productions shows similarly trend to gross water footprint,the peak appered in 2008 and animal products changed slightly; water resources efficiency and economic benefits from 2007 to 2011 were improved, but which was less than that of animal products; water resources carrying capacity index of Turkmenistan indicated that water resources utilization degree and exploring potential keep rising year by year, but still have great development potential. However, along with the rise of agriculture and husbandry water footprint, Turkmenistan is faced with abruptly water resource pressure and prominently water resource ecological security problems.