The paper focuses on the treatment of wastewater from Lac production by electro-catalytic oxidation process combined with microbial flocculation. The results indicated that advanced oxidation process can effectively remove pollutants in wastewater from Lac production, increase biodegradability and reduce the chloride ion concentration in the meantime. After 40min electro-catalytic oxidation process, CODCr removal rate reached 46.7%, CODCr removal rate combined with flocculation reached 72% while reducing the chloride concentration of 32%. The method can provide a new way for Lac production enterprise to solve the discharge of wastewater.