As a new biological nitrogen removal technology, the start-up of ANAMMOX reactor is very important. Most of researches focus on synthetic wastewater, there is rare report about its application in piggery wastewater.The culture of anaerobic oxidation biomass seeded with anaerobic denitrification sludge in the anaerobic sequencing batch reactor. It took 125days that ANAMMOX reactor was started up successfully under the 100mg/L concentration of ammonia nitrogen(NH+[KG-*2]4-N) and nitrite nitrogen(NO-[KG-*2]2-N), which was departed into initial phase of start-up, transition period and steadily operative period. At the end of the reactor start-up, the removal rate of ammonia and nitrite achieved 91.7%, 92.0% respectively; The volume loads of ammonia and nitrite were 36.9,7.6mg/(L·d) respectively.The result has important guiding significance on ANAMMOX technology in engineering practice.