A strain of sulfate reducing bacteria is separated and purified by experiment (be named SRBd), and is identified to desulfobacter preliminarily. Characteristic experiment of SRBd strain to remove Fe2+, Mn2+ in wastewater is made under different conditions of bacteria liquid dosage, pH value, oscillation intensity and carbon sulfur. The result indicated that 37℃, 6of pH, 100r / min of shock strength, 2/1of COD/SO42- are the best reaction conditions. Under the best condition, removal rate is 88.16%、99.37% and 59.18% to SO42-、Fe2+ and Mn2+ respectively. So the SRBd has the better removal performance to Fe2+, Mn2+. This result can provide a certain reference for AMD treated by biological method.