Phenol was selected as target matter to study kinetics of novel type ASBR reactor. It is a kind of difficult biodegradation organic matter. After the Acclimation of activated sludge and Orthogonal test, the experiment is operated under the conditions of pollutant degradation that is been treated most effective in ASBR reactor. The test results show that When the initial concentration of phenol is less than 146.8mg/L, the reaction rate increases with the growing of concentration of phenol, the degradation process of phenol conforms to the reaction characteristics of zero order kinetics; When the initial concentration of phenol is between the 200.5mg/L and 377.9mg/L, the reaction rate reduces with the growing of concentration of phenol, the degradation process of phenol conforms to the reaction characteristics of first order kinetics. Degradation process of phenol in low and high concentration conforms to the reaction characteristics of zero and first order kinetics, and the degradation rate at middle temperature is significantly higher than that of normal temperature. In this paper, the experimental data fitted the kinetic equation, which can provide reference conditions of treating phenol waste water in actual technology.