To explore the influence of preferential flow effect caused by different pore types on the change patterns of water content under rainfall condition, we adopted one-dimensional rainfall experiment and numerical simulation methods to analyze the water content distribution and macropore seepage characteristics of undisturbed soil and remolded soil using samples with vertical transfixion pores, dead end pores and anisotropic pores under the condition of rainfall infiltration. The results show that the water flow migrated along the soil pores from the surface to the deep layer. The shallow surface layer was dominated by uniform infiltration and the water content uniformly increased to the peak value. The preferential seepage effect was dominant in the deep layer, and the water content growth modes were different with different samples. The water content of samples with vertical transfixion pores increased slowly and the growth rate increased slightly at the later stage, because water had the priority to permeate along the side of the preferential channel, and its infiltration speed changed from slow to fast, and then the flow rapidly exited. The water in samples with dead end pores stagnated at the dead ends of the pores, so the water content of this pore type was the highest in the deep layer, where the water flow changed from vertical infiltration to lateral infiltration and the outflow was delayed. The water in anisotropic pore samples flowed through the preferential channel quickly and then diffused slowly to the other parts, the water content of the samples increased at first and then decreased, and it took a long time to migrate to the bottom of the samples and eventually flew out. The numerical simulation of dual seepage model can better describe the development characteristics and water migration laws of different macropores, and the water migration characteristics of different pore types can be characterized by setting the values of different model parameters.