The water diverted from the Yellow River is an important replenishment source for the groundwater in Yinchuan Plain. In order to analyze the response of Yinchuan Plain groundwater depth to the integrated water regulation of the Yellow River, methods such as trend analysis, R/S analysis and ArcGIS geographic spatial analysis were used to analyze the temporal and spatial variation characteristics of the groundwater depth in Yinchuan Plain from 1995 to 2019 and its main influencing factors were screened with the emphasis on the volume change of the water diversion after the implementation of the integrated water regulation of the Yellow River policy. The results show that the groundwater depth of Yinchuan Plain increased significantly from 2001 to 2019, it increased from 1.4 m in 2001 to 2.3 m in 2019. The Hurst index of the monthly average time series of groundwater depth calculated by R/S analysis method was 0.78, indicating that the dynamic series of groundwater depth was continuous. The depth of groundwater had been increasing spatially centered on Yinchuan and Shizuishan City, with the largest increase in Yinchuan City. The average annual groundwater depth of 2001-2019 was significantly correlated to the amount of water diversion from the Yellow River, and the decrease in the water diversion was the main reason for the increase in the groundwater depth. The study clarified the relationship between the depth of groundwater in Yinchuan Plain and the amount of water diversion from the Yellow River, providing a theoretical support for the promotion of ecological conservation and high quality development of the Yellow River Basin.