In order to explore the response of annual, seasonal and monthly runoff to the land use change, we chose the Juma River Basin as the study area, and established the WEP-L (water and energy transfer processes in large river basin) model of this basin. With this model, nine scenarios of land use changes were set to quantitatively analyze the influences of land use change on the runoff．The results showed that the WEP-L model was applicable to the runoff simulation of the Juma River Basin. Returning farmland to forest would reduce the annual runoff, and with the increase of forest coverage rate, the runoff decrease would be more significant. When the forest coverage rate increased by 10%, the runoff decreased by 13.10%. However, abandoned land and fire logging would increase the runoff. The response of seasonal runoff to the land use change was summer > autumn > winter > spring. The response of monthly runoff was greater from June to September, especially in July, while lower in March and April. The research results can provide a valuable reference for the planning of land use pattern in this basin.