Based on the homogenized average daily temperature observations of 34 meteorological stations in Guizhou Plateau from 1960 to 2018, the characteristics of temperature variations in Guizhou Plateau at different spatiotemporal scales was studied using the Mann-Kendall (M-K) test, Bernaola-Galavan (B-G ) algorithm, extreme point symmetric mode decomposition (ESMD) and other methods. The results show that in the past 59 years, the temperature in Guizhou Plateau has significantly increased, with an inclination rate of 0.171 0 °C/10a. Temperature abrupt changes occurred in 1997, and showed a rising trend of “non linear-non stationary-jumping”. From 1998 to 2012, the phenomenon of warming mitigation was consistent with the “stagnation” of global warming. Abrupt changes were detected in all of the four seasons. Temperatures of autumn and winter contributed the most to the annual temperature increase, whereas that of summer contributed the least. The temperature showed a rising trend in all regions spatially, among which the second step area contributed the most to the warming of the entire region. The distribution of temperature variation of four seasons was similar to that of the annual temperature variation, the temperature inclination rate all decreased from west to east in the third step area except in spring. The research results are of practical significance for the Guizhou Plateau to improve its ability in response to global warming, and can provide decision support for the rational use of hydrothermal resources and the formulation of sustainable development strategies in the region.