Through the concept of water ecological civilization, the relevant evaluation index system for Lanzhou water ecological civilization was established, and this study take Lanzhou City as an example to carry out the evaluation of water ecological civilization degree, so as to judge and solve the key problems. The improved G1 method by combining the entropy weight, coefficient of variation and G1 was used to objectively determine the weight of the evaluation index of water ecological civilization in Lanzhou City. The comprehensive evaluation method of the harmony degree equation (HDE) was used to quantitatively analyze the degree of water ecological civilization in Lanzhou City from 2014 to 2017. In the construction of water ecological civilization in Lanzhou City, the management and publicity of water supervision and water culture should be increased. The values of harmony in 2014-2017 were 0.08, 0.38, 0.72, and 0.94, respectively, indicating the degree of water ecological civilization is significantly improved. The degree of water ecological civilization in Lanzhou City has been increasing year by year. In 2017, it has reached a remarkable civilization. Through the creation of the Yellow River customs line, the water ecological construction effect in Lanzhou City has been obvious in recent years. With the different development stages, it will be accompanied by different focuses, and we should improve the indicator system and propose relevant characteristics of different time periods, to better guide the construction of water ecological civilization in Lanzhou City.