The bearing steel beam of the radial steel gate has the advantages of simple form, rapid assembly and effective reduction of lock chamber space, whose safety is directly related to the safe operation of the gate. Therefore, it is very important to design a bearing steel beam of the radial steel gate with a reasonable structure, and the stiffening rib is the important component for ensuring the local stability of the steel beam and improving the strength and rigidity. Under the condition of satisfying local stability, the reasonable interval of the transverse stiffener distance of the bearing steel beam was given by establishing the three-dimensional finite element model of different distance of the transverse stiffener of the bearing steel beam and calculating as well as analyzing the influence law on the strength and stiffness of the bearing steel beam. The result showed that the transverse stiffener distance has a great influence on the stress of the bearing steel beam flange and the stiffener itself, but has negligible effect on the web stress. When the transverse stiffener distance of the bearing steel beam was ［1/3，1/2) times the web height, the performance of the steel beam material was fully exerted, and the strength and rigidity of the bearing steel beam met the requirements of the specification, which is both economical and rational. The research results can provide reference for the design of the bearing steel beams of the radial steel gate.