The number of fish larval in the middle and lower reaches of the Yangtze River has decreased dramatically, meanwhile the time of fish spawning has delayed. In this study, the discharge requirements and ecological regulation schemes of the Three Gorges Reservoir during fish reproduction periodwere put forward according to the summarized fish reproduction conditions. And the satisfaction degree, for which the historical runoff met the ecological scheduling schemes, was calculated and analyzed. By establishing the connections between the pre-reproduction and the reproduction periods, discussing the feasibility of the pre-storage scheduling scheme when runoff can't meet the scheme. The results showed that, the inflow runoff of the Three Gorges reservoir from 2004 to 2013 had a low degree to satisf degree the ecological regulation schemes and needed to take pre-storage measures. The range of pre-storage water levels were 145.00-159.34 m under the actual inflow runoff conditions and 145.00-156.63m under the 80% probability of inflow runoff. If the reservoir pre-store the water at the 80% probability of inflow runoff, the pre-storage scheme would not increase the flood control risk of the Three Gorges Reservoir when using the forecasted 7 day pre-discharge.