The paper calculated the water footprint of the six largest-sowing-area crops in Jiangsu province between 2000 to 2010 by using water footprint theory and method.It also analyzed the characteristics of each crop's water footprint as well as the adverse effects of the gray water footprint on the water environment. The results showed that blue water and green water volume consumed in the production process of six kinds of crop generally appeared a downward trend, and water using efficiency of agricultural production has increased every year in Jiangsu Province. The green water footprint of six kinds of crop accounted for 61.88% to 88.17% of total water consumption and green water played an important role in the local crop production;the water footprint of rice and wheat production accounted for 84.15% of the total water footprint of the six major crops in Jiangsu province;the water footprint per unit mass of cotton production is the highest, and it also produced the highest gray water footprint; the water footprint per unit mass of wheat production is almost twice as well as that of rice, its gray water footprint is also significant greater than that of rice.