Through the calculation of hydrological series data before and after the completion of reservoir,the paper deeply analyzed the influence of Wuluwati reservoir construction on the Hotan river. The results show that the runoff process is out of sync in two tributaries after the completion of Wuluwati reservoir, which result in the initial flood to be consumed in the way of river and make water loss increase. In order to reasonably develop and utilize water resources in the Hotan river, the paper suggested that the layout and construction of key water control project on the Yurungkash river is necessary so as to realize joint operation of reservoirs.