Three different methods of GIS spatial interpolation and Carlson eutrophication index were applied to analyze the status and spatial distribution characteristics of eutrophication and environmental factors in Yuqiao reservoir over past 11 years. The results showed that exponential semi-variogram model of OK was the best interpolation method compared through the result of cross-validation; eutrophication of Yuqiao reservoir was mainly contributed by excessive nitrogen loading, less influenced by organic pollution, limited by phosphorus, and the spatial difference of nutrients for the reservoir was significant during flood season; eutrophication status of Yuqiao reservoir is between nutrient and mesotropher and its value is from 45.87 to 54.58, the north shore of reservoir has been in mesotropher because of livestock and poultry farming pollution and the south shore of the reservoir has become mesotropher water and tend to be of eutrophication because of agricultural non-point pollution. The results reflect the spatial characteristics of eutrophication and provide a new guideline to establish effective environmental management measures for the protection of Yuqiao reservoir.