Based on theory of sustainable development ，the paper propose evaluation model of water resources index under the typical karst ecosystem of Guizhou according to terrain of the area and the development and utilization status of water resources. After collecting and analyzing correlation data，the paper confirmed the relative weight of each index by use of mathematic means of the analytic hierarchy process，and then quantitatively evaluated the extent of sustainable utilization of water resources in Anshun.The the results show that the sustainable utilization level of water resources is 2012>2011>2010，which is evidently restricted by social environment and economic development level. However，the system can enrich the research field of water resources and make contributions to the improvement of ecological environment and the realization of sustainable utilization of resource in the karst zone of Anshun.