It is necessary to calculate the underground aquifer''s hydrogeological parameters on the evaluation and allowance of groundwater resources. The practice of pumping test in TuoKeTuo County is used for determining the confined aquifer''s hydrogeological parameters. This paper mainly discussed the feasibility of MATLAB software in optimizing the traditional Jacob liner diagram method and standard curve method based on the data of single well unsteady-flow pumping test, computed the transimissvity coefficient and permeability coefficient of the confined aquifer. The results are in accordance with the condition of local hydrologic geology and more precise and reliable than that determined by tradition method.