Based on linear regression, Mann-Kendall test and wavelet analysis methods, the temperature series from 1959to 2008in the upstream of Pearl River basin were researched. The results indicated that:①during recent 55years, the annual temperature presents a significantly upward trend. The temperature rising obviously areas focuses on Hongshui River, Liujiang River, Guijiang River, Hejiang River, Qianxunjiang River, downstream of Wuzhou and North River basin. In addition, the annual temperature exhibits 3,7, 17and 43year periods. ②As for seasonal temperature, the summer and autumn temperatures demonstrate a significantly upward trend, while there is an insignificantly upward trend in spring and winter. Generally, seasonal temperature periods are nearly as same as annual temperature, which exist more or less 3,7, 18and 40year periods. The results will play an important significance in the climate prediction of Pearl River basin.